DNA-Free mRNA Cap 2'-O-Methylt

DNA-Free mRNA Cap 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the 5´ cap structure of RNA. Using S-adenosyl methionine (SAM) as a methyl donor, it methylates capped RNA to form Cap-1. Studies have shown that the 2'-O-methylation in the Cap-1 structure can help mRNA evade immune responses in certain eukaryotic cells.

Cat. No.: DFMC2-2k (for 2KU)

Cat. No.: DFMC2-10k (for 10KU)

Description

DNA-Free mRNA Cap 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the 5´ cap structure of RNA. Using S-adenosyl methionine (SAM) as a methyl donor, it methylates capped RNA to form Cap-1. Studies have shown that the 2'-O-methylation in the Cap-1 structure can help mRNA evade immune responses in certain eukaryotic cells.

mRNA Cap 2'-O-Methyltransferase specifically methylates RNA with an m7GpppN cap structure at the 5’ end but does not methylate RNA with 5’ ends of pN, ppN, pppN, or GpppN. RNA with an m7GpppN cap structure can be obtained by adding cap analogs during in vitro transcription or using an in vitro capping system. 2 kU of mRNA Cap 2'-O-Methyltransferase can methylate 400 μg of capped RNA.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 10 pmol of an 80 nt capped RNA transcript within 60 minutes at 37℃ under 1× mRNA Cap 2'-O-Methyltransferase reaction buffer conditions.

Features

• Completely free from bacterial DNA.
• Improves mRNA expression after microinjection and transfection.
• Enhances RNA translation efficiency.