EasyBeacons for the Detection of Methylation Status of Single CpG Duplets

This chapter describes two different fast protocols for optimizing EasyBeacons™ for the challenging detection of the methylation status of a single CpG duplet in bisulfitetreated DNA. EasyBeacons™ can be used in multiplex detections even if they do not have the same affinity for their respective targets giving very specific results for both realtime polymerase chain reaction (real-time PCR) detection and endpoint analysis using inexpensive master mixes. The technology described in this chapter is a very competitive alternative to other real-time PCR technologies.